Silwet L-77 or Vac-In-Stuff for vacuum infiltration of Agrobacterium   ArabiSun light system for growing plants ArabiTray heavy-duty subirrigation tray Vivaldi bvba ARASYSTEM growing /seed harvesting system for Arabidopsis Books on Arabidopsis and Plant Molecular Biology/Genetics for sale LEHLE SEEDS Home Page
Crop seeds packaged in a convenient size for teaching cnav_crossing.gif (975 bytes) EMS mutated M2 seeds of Arabidopsis Fast Neutron mutated M2 seeds of Arabidopsis Quantum light meters & sensors for measuring photosynthetic photon flux EMS & Irradiated M2 seeds of Arabidopsis cnav_price_schedule.gif (1187 bytes)
Potting Medium for growing Arabidopsis Silwet L-77 or Vac-In-Stuff for vacuum infiltration of Agrobacterium Tobacco seeds for tissue culture and transformation Arabidopsis wild type seed strains or ecotypes Arabidopsis mutants with a visible phenotype

  GR M2

cnav_how_to_order.gif (1169 bytes)
Title: Methods in Molecular Biology 

Image of cover of Arabidopsis Protocols Second Edition

Julio Salinas and Josť J. Sanchez-Serrano

Methods in Molecular Biology 

Humana Press 

Second Edition
(March 30, 2006)
ISBN: 1-58829-395-5

Hard cover ,
Dimensions: 102 x 7.2 x 10.0 inches,
469 pages

Part I. Growing Arabidopsis. Growth of plants and preservation of seeds. Prevention and control of pests and diseases. Growth of other species related to Arabidopsis thaliana. Grafting. Synchronisation, transformation and cryopreservation of suspension cultured cells.
Part II. Genetic Anbalysis. Genetic analysis. QTL Analysis. EMS mutagenesis of Arabidopsis. Low resolution mapping of untagged mutations. Gene identification an cloning by molecular marker mapping. High-throughput TILLING for Arabidopsis. Genotyping and mapping with high density oligonucleotides arrays. Forward genetic screening of insertional mutants. PCR-based screening for insertional mutants. Cytogenetic analysis of Arabidopsis. Using information from public Arabidopsis databases to aid research. 
Part III. Transient and Stable Transformation.
In planta Agrobacterium mediated transformation by vacuum infiltration. Transient expression assay by agroinfiltration of leaves. Functional analysis of transcription factors by microparticle bombarment. Transfection assays with protoplasts containing integrated reporter genes. Chloroplast genetic engineering via organogenesis or somatic embryogenesis. b-glucuronidase as reporter gene: advantages and limitations. Use of fluorescent proteins as reporters. Luciferases as reporter genes. Aqueorin as reporter gene. Applications of chemical-inducible expression systems in functional genomics and biotechnology.
Part IV. Transcriptomics. RNA extraction Target preparation for DNA microarray hybridizaiton. Statistical issues in microarray data analysis. Preparation and quality assessment of RNA from cell-specific samples obtained by laser microdissection.
Part V. Proteomics. Protein isolation and 2-D electrophoretic separation. Isolation of nuclear proteins. Purification and fractionation of mebranes for proteomic analysis. Protein microsequencing. Peptide-based phosphoproteomics with immobilized metal ion chromatography (IMAC). 
Part VI. Metabolomics.  Metabolite profiling in Arabidopsis. Hormone profiling in Arabidopsis.

| Price Information |

Catalog Index
Catalog Home Page Our Current Price Schedule Special Notes Ordering Information LEHLE SEEDS Home Page
Wild Type Arabidopsis Seeds Vacuum Transformation Supplies   Arabidopsis Books
EMS M2 Seeds Arabidopsis Seeds for Teaching ARASYSTEM ArabiSun Lighting System
Fast Neutron M2 Seeds Tobacco Cultivars for Tissue Culture Arabidopsis Potting Medium Quantum Light Meters
Gamma-Ray and Fast Neutron M1 Seeds Crop Seeds for Teaching ArabiTray Crossing Tools
ArabiKeeper seed storage      

Return to LEHLE SEEDS Home Page

POB 2366 ROUND ROCK TX 78680-2366 USA
TEL 1-800-881-3945 or FAX 1-512-388-3974
E-Mail: /

 Revised -- June 29, 2010      Layout, design & revisions by LEHLE SEEDS     Copyright © 1995-2012, LEHLE SEEDS